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HumanZyme/Leptin/HZ-7122/200 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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HumanZyme/Leptin/HZ-7122/200 µg

ProductDescription:

Encodedbytheob(obese)gene,LeptinisanADIpose-derivedcytokinethatsuppressesappetiteandincreasesThermogenesis.LeptinexertsitsanorecticeffectviasignalingthroughahypothalamicreceptortermedOB-R.Leptinhasbeenshowntoreducebodyweight,foodconsumption,andplasmaglucoselevelsinvariousinvivomodels.RecombinantHumanLeptinisa16.0kDaproteincontaining147aminoacidresidues.

TechnicalSpecifications:

  • Species:Human
  • Expression:E.coliCellExpressed
  • Purity:≥98%
  • Endotoxin:<1.0EU/μg
  • MolecularMass:16.0kDa
  • CountryofOrigin:USA

PurityConfirmation:

ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.

ActivityAssay:

Determinedbyaninvivoassayusingtheob/obmouseobesitymodelandNZO.Bothstrainsofmiceweretreatedviaintraperitonealinjectiononcedailyatadoseof5μgleptin/gmofbodyweightfor7days.Significanteffectsonbodyweight,foodconsumption,andplasmaglucoselevelswereobservedtosaline-treatedcontrols.

AASequence:

MVPIQKVQDDTKTLIKTIVTRINDISHTQS
VSSKQKVTGLDFIPGLHPILTLSKMDQTLA
VYQQILTSMPSRNVIQISNDLENLRDLLHV
LAFSKSCHLPWASGLETLDSLGGVLEASGY
STEVVALSRLQGSLQDMLWQLDLSPGC

ReconstitutionBuffer:

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof1.0-5.0mg/ml.Donotvortex.

Storage:

Thissolutioncanbestoredat2-8°Cforupto1week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.

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