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PhosphoSolutions/Glycine Receptor Antibody/700-GLY/200 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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产品资料

PhosphoSolutions/Glycine Receptor Antibody/700-GLY/200 µg

Rabbitpolyclonalantibody

PooledSerum
Size:
200µg
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human
Applications:
WB1:1,000IHC1:1,000
Species:
Rabbit
MolecularReference:
~48kDa
CiteThisAntibody:
PhosphoSolutionsCat#700-GLY,RRID:AB_2492133
Antigen/Purification:ExpandCollapse

TheantigenisapeptidefromtheN-terminusregionoftheα1-subunitofratglycinereceptor.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.

BIOLOGicalSignificance:ExpandCollapse

Glycineisanimportantinhibitorytransmitterinthebrainstemandspinalcord.Glycinereceptorsaremembersoftheligand-gatedionchannelfamily(LGICs)thatmediaterapidchemicalneurotransmission(Schofieldetal.,2003).Thebindingofglycinetoitsreceptorproducesalargeincreaseinchlorideconductance,whichcausesmembranehyperpolarization.Glycinereceptorsareanchoredatinhibitorychemicalsynapsesbyacytoplasmicprotein,gephyrin(Fischeretal.,2000).TheglycinereceptorhasbeenusedtogreatadvantageintheidentificationofthebindingsitesforalcoholontheLGICfamilyofproteins(Becksteadetal.,2001;Mihicetal.,1997).Thesereceptorshavealsobeenextremelyusefulinstudiesofsynapticclusteringofreceptors(CraigandLichtman,2001).TheglycinereceptormayalsoactinconcertwithanNMDARsubunittoformanexcitatoryreceptor(Chattertonetal.,2002).

Storage

200µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

ProductSpecificReferences

WickMJ,BleckV,WhatleyVJ,BrozowskiSJ,NixonK,CardosoRA,andValenzuelaCF(1999)Stableexpressionofhumanglycinealpha1andalpha2homomericreceptorsinmouseL(tk-)cells.JNeurosciMethods87(1):97-103.PMID:10065988

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