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PhosphoSolutions/Potassium Chloride Cotransporter 2 (KCC2) (Thr1007) Antibody/p1551-1007/100 µl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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PhosphoSolutions/Potassium Chloride Cotransporter 2 (KCC2) (Thr1007) Antibody/p1551-1007/100 µl

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Bovine,Human,Mouse,Non-humanPrimate,Pig,Rat,Sheep
Applications:
WB1:1000
Species:
Rabbit
MolecularReference:
~135kDa
CiteThisAntibody:
PhosphoSolutionsCat#p1551-1007,RRID:AB_2716769
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr1007ofmouseKCC2.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-andnon-phosphopeptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

KCC2iswidelythoughttobeexpressedexclusivelyinneuronswhereitisresponsIBLeformaintaininglowintracellularchlorideconcentrationtodrivehyperpolarizingpost-synapticresponsestotheinhibitoryneurotransmittersGABAandglycine(Leeetal.,2007).KCC2isexpressedinmostadultneurons,andexpressionlevelscorrelatewellwiththematurationstateofneurons.N-ethylmaleimide(NEM)hasbeenshowtoincreasephosphorylationoftheSer940residue,whiledecreasingphosphorylationofT1007residue.DephosphorylationofresiduesT906andT1007correlateswithincreasedKCC2activity(Mossetal.,2017).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

ProductSpecificReferences

Conway,L.C.,Cardarelli,R.A.,Moore,Y.E.,Jones,K.,McWilliams,L.J.,Baker,D.J.,Burnham,M.P.,Bürli,R.W.,Wang,Q.,Brandon,N.J.andMoss,S.J.,2017.N-ethylmaleimideincreasesKCC2activitybymodulatingtransporterphosphorylation.JournalofBiologicalChemistry,pp.jbc-M117.

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