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PhosphoSolutions/MARCKS (Ser152,156) Antibody/p1185-1526/100 µl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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PhosphoSolutions/MARCKS (Ser152,156) Antibody/p1185-1526/100 µl

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human,Xenopus,Bovine,Chicken,Zebrafish
Applications:
WB1:1,000
Species:
Rabbit
MolecularReference:
~87kDa
CiteThisAntibody:
PhosphoSolutionsCat#p1185-1526,RRID:AB_2492143
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser152,156ofMARCKS.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

MyristoylatedAlanine-RichCKinaseSubstrate(MARCKS)isamajorsubstrateforphosphorylationbyproteinkinaseC(PKC)(Ouimetetal.,1990).ThephosphorylationofSer152/156canbeusedasameasureofPKCactivationalthoughthesesitesarealsophosphorylatedbyPRK1(Palmeretal.,1996)MARCKSisamemberofafamilyofcalmodulinbindingproteinsandphosphorylationofSer152/156modulatesthebindingofMARCKStocalmodulin(Vergheseetal.,1994)

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

OuimetCC,WangJKT,WalaasSI,AlbertKA,GreengardP(1990)LocalizationoftheMARCKS(87kDa)protein,amajorspecificsubstrateforproteinkinaseC,inratbrain.JNeurosci10:1683-1698.

PalmerRH,SchönwasserDC,RahmanD,PappinDJC,HergetT,ParkerPJ(1996)PRK1phosphorylatesMARCKSatthePKCsites:Serine152,serine156andserine163.FEBSLett378:281-285.

VergheseGM,JohnsonJD,VasulkaC,HauptDM,StumpoDJ,BlackshearPJ(1994)ProteinkinaseC-mediatedphosphorylationandcalmodulinbindingofrecombinantmyristoylatedalanine-richCkinasesubstrate(MARCKS)andMARCKS-relatedprotein.JBiolChem269:9361-9367.

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