产品资料

Rockland/Apolipoprotein J Antibody/600-101-198/500 µg

ApolipoproteinJAntibodyProperties

Description

Anti-ApolipoproteinJ(GOAT)Antibody-600-101-198

Host

Goat

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

ELISA:1:5,000-1:10,000
WesternBlot:1:5,000-1:10,000
Immunohistochemistry:1:50-1:200
ImmunoPrecipitation:1:100
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

WetIce

Format

IgG

Label

Unconjugated

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.125MSodiumBorate,0.075MSodiumChloride,0.005MEDTA,pH8.0

Preservative

0.01%(w/v)SodiumAzide

StABIlizer

None

GeneName

CLU,APOJ,CLI,KUB1

RelevantLinks

UniProtKB-P10909
NCBI-P10909.1
GeneID-1191

ApolipoproteinJAntibodyDescription

Background

ApolipoproteinJAntibodyfunctionsasasecretedchaperonethatpreventsaggregationofnonnativeproteins.Itpreventsstress-inducedaggregationofbloodplasmaproteinsandinhibitstheformationsofamyloidfibrils.ApolipoproteinJdoesnotrequireATPorrefoldproteinsbyitself.Itmaintainspartiallyunfoldedproteinsinastateforsubsequentrefoldingbyotherchaperones.ItisshowntobeinvolvedinseveralbasicBIOLOGicaleventssuchascelldeath,tumorprogression,andneurodegenerativedisorders.Bindingtocellsurfacereceptorsittriggersinternalizationofchaperone-clientcomplexandsubsequentlysosomalorproteasomaldegradation.ItmodulatesNF-kappa-Btranscriptionalactivity.NuclearisoformspromoteapoptosiswhilemitochondrialisoformssuppressBAX-dependentreleaseofcytochromecintothecytoplasmandinhibitapoptosis.Anti-ApolipoproteinJAntibodyisusefulforresearchersinterestedintheimmunesystem,Ubiquitinpathways,andcardiovascularresearch.

Synonyms

goatanti-ApolipoproteinJantibody,Clusterin,Complement-associatedproteinSP-40,Complementcytolysisinhibitor,CLI,NA1/NA2,Apo-J,Testosterone-repressedprostatemessage2,TRPM-2,Ku70-bindingprotein1,Aging-associatedgene4protein,Clusterinbetachain,Clusterinalphachain,Complementcytolysisinhibitorachain,Complementcytolysisinhibitorbchain,ApoJalpha,ApoJbeta,CLU,CLI,KUB1,AAG4

Immunogen

apoLipoproteinTypeJwasisolatedfromhumanplasmabydensitygrADIentcentrifugationfollowedbyHPLCpurification.

ImmunogenType

NativeProtein

StorageCondition

Storevialat4°Cpriortoopening. Thisproductisstableat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse. Forextendedstoragemixwithanequalvolumeofglycerol,aliquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing.

ApplicationNote

Anti-apolipoproteinantibodieshavebeenusedforindirecttrappingELISAforquantitationofantigeninserumusingastandardcurve,forimmunoprecipitationandforwesternblottingforhighlysensitivequalitativeanalysis.

Purity/Specifity

Thisproducthasbeenpreparedbyimmunoaffinitychromatographyusingimmobilizedantigensfollowedbyextensivecross-adsorptionagainstotherapoLipoproteinsandhumanserumproteinstoremoveanyunwantedspecificities. Typicallylessthan1%crossreactivityagainstothertypesofapoLipoproteinwasdetectedbyELISAagainstpurifiedstandards. ThisantibodyreactswithhumanapoLipoproteinJandhasnegligIBLecross-reactivitywithTypeA-I,A-II,B,C-I,C-II,C-IIIandEapoLipoproteins. Specificcrossreactionofanti-apoLipoproteinantibodieswithantigensfromotherspecieshasnotbeendetermined. Non-specificcrossreactionofanti-apoLipoproteinantibodieswithotherhumanserumproteinsisnegligible.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Apolipoprotein J Antibody/600-101-198/500 µg

ApolipoproteinJAntibodyProperties

ApolipoproteinJAntibodyDescription