产品资料

Rockland/AKT3 PE Antibody/200-308-E75/50 µg

AKT3PEAntibodyProperties

Description

Anti-AKT3(MOUSE)PhycoerythrinConjugatedMonoclonalAntibody-200-308-E75

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

Human,Mouse,Rat

Clonality

Monoclonal25F6.F6.D8IgG1kappa

Application

ELISA:UserOptimized
IFMicroscopy:Useroptimized
FlowCytometry:UserOptimized
WesternBlot:UserOptimized
Immunohistochemistry:Useroptimized

PhysicalState

Lyophilized

ShippingCondition

WetIce

Format

IgG1

Label

R-Phycoerythrin(RPE)

FPValue

1-2molesR-Phycoerythrin(RPE)permoleofIgG1

Concentration

1mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2

ReconstitutionVolume

50µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

None

StABIlizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

AKT3

RelevantLinks

UniProtKB-Q9Y243
NCBI-NP_001193658.1
GeneID-10000

AKT3PEAntibodyDescription

Background

AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT3(MOUSE)PEconjugatedMonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.

Synonyms

Mouseanti-AKT3antibodyPEconjugation,phycoerythtinconjugatedMouseanti-AKT3antibody,AKT-3,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt3antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma

Immunogen

Anti-AKT3AntibodywaspreparedfromtissueculturesupernatantbyProteinAaffinitychromatographyusingasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT3protein.

ImmunogenType

Peptide

StorageCondition

Storevialat4°Cpriortorestoration.Restorewithdeionizedwater(orequivalent).Thisproductisstableat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Donotfreezeafterreconstitution.Storereagentinthedark.UsesuBDuedlightingduringhandlingandincubationofcellspriortoanalysis.

ApplicationNote

Anti-AKT3PEAntibodyissuitableforFlowCytometry,ELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT3proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.

Purity/Specifity

Anti-AKT3antibodyisdirectedagainsthumanAKT3.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT3antibodywaspurifiedfromtissueculturebyProteinAchromatography.CrossreactivitywithAKT3fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows100%identitytohuman,mouse,andrat,therefore,crossreactivityisexpected.Cross-reactivitywithAKT2andAKThasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/AKT3 PE Antibody/200-308-E75/50 µg

AKT3PEAntibodyProperties

AKT3PEAntibodyDescription