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| Description | Nervegrowthfactor(NGF)issynthesizedasaprecursor(proNGF)whichmaybereleasedandhavephysiologicalfunctionstocausecelldeath.Itbindsneurotrophinreceptorp75andsortilinandmayalsobeimportantforthedevelopmentofnervoussystem.proNGFissynthesizedintargettissuesandglia,transportedretrogradelyandmaybereleased. |
| Relatedproducts | BiotinylatedproNGFantibody,cat#M-1778-50-B |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(C-HTIPQAHWTKLQ,aa:30-41)ofhumanproNGFproteinhasbeenusedastheimmunogen.Thesequenceislocatedonthepro-domainoftheproNGFfull-lengthproteinandis80%homologoustomouseandratproNGF. |
| AntibodyType | MousemonoclonalIgGantibody |
| Isotype | IgG2b,lamBDa-lightchain |
| OtherNames | Pro-brainnervegrowthfactor;proNGF;NGF |
| Accession | NGF_HUMAN |
| Producedin | Mouse |
| Purity | ProteinGpurifiedmouseIgG. |
| Applications | FlowCytometry(2µg/10^6cells).Immunocytochemistry(1-2ug/mL),WesternBlotting(1-2ug/mL).Otherapplicationsnotyettested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Human |
| Cross-reactivity | Speciescross-reactivitynottested. |
| Form | LyophilisedfromasolutioncontainingPBSbufferpH7.4with3%trehalose,withoutpreservatives. |
| Reconstitution | Spinvialbrieflybeforeopening.Reconstitutein100µLofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservatives. |
| Storage | Storelyophilizedantibodyat2-8ºC.Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstABIlity.Antibodycontainsnopreservatives.Storeat4ºCwithanappropriateantibacterialagent.Usesterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened |
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