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| Description | Cytokeratin8isatypeIIkeratinandamemberofintermediatefilamentfamilyofproteins.Cytokeratin8typicallyformsaheterotetramerwithCytokeratin18toformanintermediatefilamentinsimplesingle-layeredepithelialcells. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(QQQKTARSNMDNMFESYIN)correspondingtoaregion(127-145)fromhumanCytokeratin8.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
| OtherNames | Keratin;typeIIcytoskeletal8;Cytokeratin-8;CK-8;Keratin-8;K8;KRT8;CYK8; |
| Accession | P05787K2C8_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0µg/mlisrecommendedforWB.HumanCytokeratin8hasapredictedlengthof483aminoacidsandMWof54kDa.Aconcentrationof2.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
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