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| Description | The mouse Interleukin 10 (IL-10) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-10 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-10 concentration are added to these wells and allowed to complex with the bound IL-10 antibody. A biotinylated mouse IL-10 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-10 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-10 in samples such as sera and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Other Names | Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; |
| Accession | P18893 IL10_MOUSE; |
| Specificity | Natural and recombinant Mouse IL-10 |
| Storage | Store at 4°C |
| Kit components | The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 15.6 pg/ml - 1,000 pg/ml |
| Sensitivity | < 1 pg/ml |
| Kit protocol |  BEK-2046-2P_as_at_Jun2017_protocol.pdf |
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